Development And Characterization Of Modified Chitosan Lipopolyplex For An Effective SiRNA Delivery
Cytotoxicity, speedy degradation, and limited cellular absorption are the foremost characteristics regulating the successful delivery of RNAs. chitosan price (Cs) is a polymer that offers an advantage due to its bio-compatibility and biodegradable nature, making it an ideal polycationic vector for delivering siRNA. In this study, chitosan has been changed with arginine in order to increase its encapsulation of siRNA and improve cellular absorption. It was revealed that arginine and guanidino medietys could transport through membranes of cells and play an important part in membrane permeability. FTIR and (13)C NMR were used to characterize the complex. These chitosan-arginine (CsAr) siRNA composites are further encapsulated in anionic DPPC/cholesterol liposomes to combine the events of liposome-chitosan-arginine complexes telephoned lipopolyplexes (LCAr).
forged LCAr were investigated for their lipid/CsAr-siRNA ratios, size, zeta-potential, heparin, and serum RNase stability by agarose gel retardation, and cell uptake efficiency compared to their "parent" polyplexes. resultants uncovered complete lipidation of CsAr-siRNA polyplexes at lipid mass ratio 10 leading in lipopolyplexes in the 120 to 230nm range. Polyplex trammeled ~70% of siRNA, whereas lipidation increases siRNA encapsulation to ~95%. Developed chitosan price exhibited ~4 times less hemolytic potential as likened to the parent polyplexes at the highest siRNA dose. The CsAr-siRNA and its lipid-coated form demonstrated enhanced cellular association as compared to the marketed Lipofectamine 2000 testifying its effectiveness in siRNA delivery. CsAr-liposome conjugation is simple and safe, and functions as a robust carrier for gene transport in physiological offices without compromising transfection efficacy.Chitosan grinded hybrid superabsorbent for contained drug delivery application.
In the present study, a hybrid chitosan-alginate superabsorbent is groomed utilizing maleic acid as a cross-linker and acrylamide as a grafting agent habituating the free radical mechanism. The composite hydrogel ushers good swelling capacity along with hemocompatibility and biocompatibility and hence it is employed as a drug delivery device. The characterization techniques including x-ray diffraction, Fourier transform infrared, x-ray photoelectron spectroscopy, and thermal analysis indicate the successful synthesis of stable hydrogel with rich functionalities. Metformin hydrochloride is used as a model drug which is used to treat diabetes. The drug encapsulation is done utilizing the tumefying diffusion method after the synthesis of hydrogel. The release of metformin from the drug-loaded hydrogel at physiological pH highlightings the role of non-covalent interactions between the drug and hydrogel. In vitro release fields of Metformin from the drug-stretched hydrogel show higher release profiles at intestinal pH (7) compared to stomach pH (1).
The observed cumulative release is 82% at pH 7 and 45% at pH 1 after 10 h. Brunauer-Emmett-Teller analysis divulges the effect of surface area, pore size, and pore volume of hydrogel on the drug release. The drug release from the hybrid chitosan-alginate hydrogel is regained to be more sustained in comparison to the pure chitosan hydrogel. For the present drug delivery system, the swelling-controlled release is found to be more dominating than the pH-moderated release. The synthesized hydrogel can be successfully applyed as a potential drug delivery system for assured drug delivery.Chitosan as a Control Tool for Insect Pest Management: A Review.Chitosan, a polysaccharide infered from the deacetylation of chitin, is a versatile and eco-friendly biopolymer with several applications.
Chitosan is accredited for its biodegradability, biocompatibility, and non-toxicity, beyond its antimicrobial, antioxidant, and antitumoral activenessses. Thanks to its properties, chitosan is used in many battlegrounds admiting medicine, pharmacy, cosmetics, textile, nutrition, and agriculture. This review centres on chitosan's role as a tool in insect pest control, particularly for agriculture, foodstuff, and public health blighters. Different conceptualisations, admiting plain chitosan, chitosan coating, chitosan with nematodes, chitosan's modifications, and chitosan nanoparticles, are explored.